tyr204 p erk 1 2 Search Results


heat shock protein 47 antibody 10811611 novus biologicals mab9166  (Novus Biologicals)
93
Novus Biologicals heat shock protein 47 antibody 10811611 novus biologicals mab9166
Antibodies Used in This Study
Heat Shock Protein 47 Antibody 10811611 Novus Biologicals Mab9166, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/heat shock protein 47 antibody 10811611 novus biologicals mab9166/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
heat shock protein 47 antibody 10811611 novus biologicals mab9166 - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
phospho specific anti erk1 2 thr202 tyr204  (R&D Systems)
92
R&D Systems phospho specific anti erk1 2 thr202 tyr204
Antibodies Used in This Study
Phospho Specific Anti Erk1 2 Thr202 Tyr204, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phospho specific anti erk1 2 thr202 tyr204/product/R&D Systems
Average 92 stars, based on 1 article reviews
phospho specific anti erk1 2 thr202 tyr204 - by Bioz Stars, 2026-02
92/100 stars
  Buy from Supplier

Image Search Results


Antibodies Used in This Study

Journal: The American Journal of Pathology

Article Title: Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation

doi: 10.1016/j.ajpath.2017.11.017

Figure Lengend Snippet: Antibodies Used in This Study

Article Snippet: Western blots were quantified by densitometry using the ImageJ densitometry program (NIH). table ft1 table-wrap mode="anchored" t5 caption a7 Name Citation (PubMed ID) Supplier (location) Catalog number Clone number Anti-AKAP12 antibody 25188285 Abcam (Cambridge, MA) ab49849 JP74 Antiphosphoserine antibody 19383294 Abcam ab9332 Polyclonal IgG Antiphosphotyrosine antibody 26310847 Abcam ab9319 Polyclonal IgG Anti–α-smooth muscle actin antibody 20043323 Abcam ab5694 Polyclonal IgG Anti-PKCα antibody Genetex (Irvine, CA) GTX130453 Polyclonal IgG Anti–cyclin D1 antibody 25956904 Abcam Ab10540 DCS-6 Anti–collagen Iα (1) antibody 25294683 Novus Biologicals (Littleton, CO) NB600-450 COL-1 Anti–heat shock protein 47 antibody 10811611 Novus Biologicals MAB9166-100 950806 Anti–phospho-p44/p42 MAPK (ERK1/2) (Thr202/Tyr204) antibody 28220783 Cell Signaling Technologies (Danvers, MA) 9101 Polyclonal IgG Anti–β-actin−peroxidase antibody (control for in vitro experiments) 27831566 Sigma (St. Louis, MO) A3854 AC-15 Anti-GAPDH antibody (control for in vivo experiments) 28761447 Cell Signaling Technologies 2118 14C10 Anti-desmin antibody 23308088 Dako (Agilent; Santa Clara, CA) M0760 D33 Open in a separate window AKAP, A-kinase anchoring protein; ERK, extracellular signal–regulated kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ID, identification; MAPK, mitogen-activated protein kinase; PKC, protein kinase C. Antibodies Used in This Study Coimmunoprecipitation Coimmunoprecipitation of proteins from cells or tissue extracts was performed, as published by us previously.

Techniques: In Vitro, In Vivo

Proteins Cross-Linked to AKAP12 in Normal HSCs

Journal: The American Journal of Pathology

Article Title: Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation

doi: 10.1016/j.ajpath.2017.11.017

Figure Lengend Snippet: Proteins Cross-Linked to AKAP12 in Normal HSCs

Article Snippet: Western blots were quantified by densitometry using the ImageJ densitometry program (NIH). table ft1 table-wrap mode="anchored" t5 caption a7 Name Citation (PubMed ID) Supplier (location) Catalog number Clone number Anti-AKAP12 antibody 25188285 Abcam (Cambridge, MA) ab49849 JP74 Antiphosphoserine antibody 19383294 Abcam ab9332 Polyclonal IgG Antiphosphotyrosine antibody 26310847 Abcam ab9319 Polyclonal IgG Anti–α-smooth muscle actin antibody 20043323 Abcam ab5694 Polyclonal IgG Anti-PKCα antibody Genetex (Irvine, CA) GTX130453 Polyclonal IgG Anti–cyclin D1 antibody 25956904 Abcam Ab10540 DCS-6 Anti–collagen Iα (1) antibody 25294683 Novus Biologicals (Littleton, CO) NB600-450 COL-1 Anti–heat shock protein 47 antibody 10811611 Novus Biologicals MAB9166-100 950806 Anti–phospho-p44/p42 MAPK (ERK1/2) (Thr202/Tyr204) antibody 28220783 Cell Signaling Technologies (Danvers, MA) 9101 Polyclonal IgG Anti–β-actin−peroxidase antibody (control for in vitro experiments) 27831566 Sigma (St. Louis, MO) A3854 AC-15 Anti-GAPDH antibody (control for in vivo experiments) 28761447 Cell Signaling Technologies 2118 14C10 Anti-desmin antibody 23308088 Dako (Agilent; Santa Clara, CA) M0760 D33 Open in a separate window AKAP, A-kinase anchoring protein; ERK, extracellular signal–regulated kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ID, identification; MAPK, mitogen-activated protein kinase; PKC, protein kinase C. Antibodies Used in This Study Coimmunoprecipitation Coimmunoprecipitation of proteins from cells or tissue extracts was performed, as published by us previously.

Techniques: Sequencing

Ethanol exposure inhibits the interaction of AKAP12 with the collagen chaperone, heat shock protein 47 (HSP47), in hepatic stellate cells (HSCs). A: Control or ethanol-treated HSCs were immunoprecipitated (IP) with AKAP12 or collagen and then Western blotted (WB) for HSP47 or collagen (Materials and Methods). B: IP blot: Phosphorylated and unphosphorylated fractions of control or ethanol-treated HSCs were purified using Qiagen columns (Materials and Methods) and subjected to immunoprecipitation with HSP47 antibody, followed by Western blotting for AKAP12. Phos-tag blot: AKAP12 phosphorylated (P-AKAP) and unphosphorylated Qiagen fractions from control or ethanol-treated HSCs were compared on Phos-tag gels. Phosphorylated extracellular signal–regulated kinase (p-ERK) was a positive control and is found mainly in the phosphorylated fraction. C: Control or ethanol-fed mouse livers were immunoprecipitated with HSP47 antibody followed by Western blotting for AKAP12. Three experiments are shown. D: Normal or alcoholic hepatitis liver extracts were immunoprecipitated with HSP47 antibody, followed by Western blotting for AKAP12. Experiments were performed in duplicate. Data are expressed as means ± SEM (A–D); n = 5 experiments (A and C); n = 3 experiments (B and D). ∗P < 0.05, ∗∗P < 0.01 versus control; †P < 0.05, ††P < 0.01, and ††††P < 0.001 versus unphosphorylated control; ‡‡‡‡P < 0.001 versus pair-fed control; §§§§P < 0.001 versus normal. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Journal: The American Journal of Pathology

Article Title: Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation

doi: 10.1016/j.ajpath.2017.11.017

Figure Lengend Snippet: Ethanol exposure inhibits the interaction of AKAP12 with the collagen chaperone, heat shock protein 47 (HSP47), in hepatic stellate cells (HSCs). A: Control or ethanol-treated HSCs were immunoprecipitated (IP) with AKAP12 or collagen and then Western blotted (WB) for HSP47 or collagen (Materials and Methods). B: IP blot: Phosphorylated and unphosphorylated fractions of control or ethanol-treated HSCs were purified using Qiagen columns (Materials and Methods) and subjected to immunoprecipitation with HSP47 antibody, followed by Western blotting for AKAP12. Phos-tag blot: AKAP12 phosphorylated (P-AKAP) and unphosphorylated Qiagen fractions from control or ethanol-treated HSCs were compared on Phos-tag gels. Phosphorylated extracellular signal–regulated kinase (p-ERK) was a positive control and is found mainly in the phosphorylated fraction. C: Control or ethanol-fed mouse livers were immunoprecipitated with HSP47 antibody followed by Western blotting for AKAP12. Three experiments are shown. D: Normal or alcoholic hepatitis liver extracts were immunoprecipitated with HSP47 antibody, followed by Western blotting for AKAP12. Experiments were performed in duplicate. Data are expressed as means ± SEM (A–D); n = 5 experiments (A and C); n = 3 experiments (B and D). ∗P < 0.05, ∗∗P < 0.01 versus control; †P < 0.05, ††P < 0.01, and ††††P < 0.001 versus unphosphorylated control; ‡‡‡‡P < 0.001 versus pair-fed control; §§§§P < 0.001 versus normal. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

Article Snippet: Western blots were quantified by densitometry using the ImageJ densitometry program (NIH). table ft1 table-wrap mode="anchored" t5 caption a7 Name Citation (PubMed ID) Supplier (location) Catalog number Clone number Anti-AKAP12 antibody 25188285 Abcam (Cambridge, MA) ab49849 JP74 Antiphosphoserine antibody 19383294 Abcam ab9332 Polyclonal IgG Antiphosphotyrosine antibody 26310847 Abcam ab9319 Polyclonal IgG Anti–α-smooth muscle actin antibody 20043323 Abcam ab5694 Polyclonal IgG Anti-PKCα antibody Genetex (Irvine, CA) GTX130453 Polyclonal IgG Anti–cyclin D1 antibody 25956904 Abcam Ab10540 DCS-6 Anti–collagen Iα (1) antibody 25294683 Novus Biologicals (Littleton, CO) NB600-450 COL-1 Anti–heat shock protein 47 antibody 10811611 Novus Biologicals MAB9166-100 950806 Anti–phospho-p44/p42 MAPK (ERK1/2) (Thr202/Tyr204) antibody 28220783 Cell Signaling Technologies (Danvers, MA) 9101 Polyclonal IgG Anti–β-actin−peroxidase antibody (control for in vitro experiments) 27831566 Sigma (St. Louis, MO) A3854 AC-15 Anti-GAPDH antibody (control for in vivo experiments) 28761447 Cell Signaling Technologies 2118 14C10 Anti-desmin antibody 23308088 Dako (Agilent; Santa Clara, CA) M0760 D33 Open in a separate window AKAP, A-kinase anchoring protein; ERK, extracellular signal–regulated kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ID, identification; MAPK, mitogen-activated protein kinase; PKC, protein kinase C. Antibodies Used in This Study Coimmunoprecipitation Coimmunoprecipitation of proteins from cells or tissue extracts was performed, as published by us previously.

Techniques: Immunoprecipitation, Western Blot, Purification, Positive Control

AKAP12 regulates the ability of collagen to interact with heat shock protein 47 (HSP47). A: Hepatic stellate cells (HSCs) transfected with AKAP12 or negative control siRNA immunoprecipitated (IP) with collagen antibody, followed by HSP47 immunoblotting. B: HSCs transfected with AKAP12 vector or empty vector control were immunoprecipitated with collagen antibody, followed by HSP47 Western blotting (WB). Data are expressed as means ± SEM (A and B). n = 5 experiments (A and B). ∗P < 0.05, ∗∗∗∗P < 0.001 versus negative control; ††P < 0.01, ††††P < 0.001 versus empty vector.

Journal: The American Journal of Pathology

Article Title: Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation

doi: 10.1016/j.ajpath.2017.11.017

Figure Lengend Snippet: AKAP12 regulates the ability of collagen to interact with heat shock protein 47 (HSP47). A: Hepatic stellate cells (HSCs) transfected with AKAP12 or negative control siRNA immunoprecipitated (IP) with collagen antibody, followed by HSP47 immunoblotting. B: HSCs transfected with AKAP12 vector or empty vector control were immunoprecipitated with collagen antibody, followed by HSP47 Western blotting (WB). Data are expressed as means ± SEM (A and B). n = 5 experiments (A and B). ∗P < 0.05, ∗∗∗∗P < 0.001 versus negative control; ††P < 0.01, ††††P < 0.001 versus empty vector.

Article Snippet: Western blots were quantified by densitometry using the ImageJ densitometry program (NIH). table ft1 table-wrap mode="anchored" t5 caption a7 Name Citation (PubMed ID) Supplier (location) Catalog number Clone number Anti-AKAP12 antibody 25188285 Abcam (Cambridge, MA) ab49849 JP74 Antiphosphoserine antibody 19383294 Abcam ab9332 Polyclonal IgG Antiphosphotyrosine antibody 26310847 Abcam ab9319 Polyclonal IgG Anti–α-smooth muscle actin antibody 20043323 Abcam ab5694 Polyclonal IgG Anti-PKCα antibody Genetex (Irvine, CA) GTX130453 Polyclonal IgG Anti–cyclin D1 antibody 25956904 Abcam Ab10540 DCS-6 Anti–collagen Iα (1) antibody 25294683 Novus Biologicals (Littleton, CO) NB600-450 COL-1 Anti–heat shock protein 47 antibody 10811611 Novus Biologicals MAB9166-100 950806 Anti–phospho-p44/p42 MAPK (ERK1/2) (Thr202/Tyr204) antibody 28220783 Cell Signaling Technologies (Danvers, MA) 9101 Polyclonal IgG Anti–β-actin−peroxidase antibody (control for in vitro experiments) 27831566 Sigma (St. Louis, MO) A3854 AC-15 Anti-GAPDH antibody (control for in vivo experiments) 28761447 Cell Signaling Technologies 2118 14C10 Anti-desmin antibody 23308088 Dako (Agilent; Santa Clara, CA) M0760 D33 Open in a separate window AKAP, A-kinase anchoring protein; ERK, extracellular signal–regulated kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ID, identification; MAPK, mitogen-activated protein kinase; PKC, protein kinase C. Antibodies Used in This Study Coimmunoprecipitation Coimmunoprecipitation of proteins from cells or tissue extracts was performed, as published by us previously.

Techniques: Transfection, Negative Control, Immunoprecipitation, Western Blot, Plasmid Preparation

Summary of mechanisms of action of phospho-AKAP12 in alcoholic liver injury. In normal liver, AKAP12 acts as a scaffold protein for protein kinase C (PKCα) and cyclin-D1 (CCND1). Both hepatocytes and hepatic stellate cells (HSCs) exhibit these functions. Normal HSCs also exhibit specific interaction of AKAP12 with the collagen chaperone, heat shock protein 47 (HSP47), and through this interaction, they might regulate collagen maturation and secretion. During alcoholic liver injury, there is decreased interaction of AKAP12 with PKCα and CCND1 in HSCs because of enhanced AKAP12 phosphorylation and loss of its scaffolding activity. Loss of this scaffolding activity may result in changes in HSC proliferation, cytokinesis, and altered signaling. HSC-specific scaffolding of HSP47 is lost when AKAP12 is phosphorylated on alcohol exposure, and this may facilitate HSP47-collagen chaperoning that may further induce collagen maturation and secretion.

Journal: The American Journal of Pathology

Article Title: Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation

doi: 10.1016/j.ajpath.2017.11.017

Figure Lengend Snippet: Summary of mechanisms of action of phospho-AKAP12 in alcoholic liver injury. In normal liver, AKAP12 acts as a scaffold protein for protein kinase C (PKCα) and cyclin-D1 (CCND1). Both hepatocytes and hepatic stellate cells (HSCs) exhibit these functions. Normal HSCs also exhibit specific interaction of AKAP12 with the collagen chaperone, heat shock protein 47 (HSP47), and through this interaction, they might regulate collagen maturation and secretion. During alcoholic liver injury, there is decreased interaction of AKAP12 with PKCα and CCND1 in HSCs because of enhanced AKAP12 phosphorylation and loss of its scaffolding activity. Loss of this scaffolding activity may result in changes in HSC proliferation, cytokinesis, and altered signaling. HSC-specific scaffolding of HSP47 is lost when AKAP12 is phosphorylated on alcohol exposure, and this may facilitate HSP47-collagen chaperoning that may further induce collagen maturation and secretion.

Article Snippet: Western blots were quantified by densitometry using the ImageJ densitometry program (NIH). table ft1 table-wrap mode="anchored" t5 caption a7 Name Citation (PubMed ID) Supplier (location) Catalog number Clone number Anti-AKAP12 antibody 25188285 Abcam (Cambridge, MA) ab49849 JP74 Antiphosphoserine antibody 19383294 Abcam ab9332 Polyclonal IgG Antiphosphotyrosine antibody 26310847 Abcam ab9319 Polyclonal IgG Anti–α-smooth muscle actin antibody 20043323 Abcam ab5694 Polyclonal IgG Anti-PKCα antibody Genetex (Irvine, CA) GTX130453 Polyclonal IgG Anti–cyclin D1 antibody 25956904 Abcam Ab10540 DCS-6 Anti–collagen Iα (1) antibody 25294683 Novus Biologicals (Littleton, CO) NB600-450 COL-1 Anti–heat shock protein 47 antibody 10811611 Novus Biologicals MAB9166-100 950806 Anti–phospho-p44/p42 MAPK (ERK1/2) (Thr202/Tyr204) antibody 28220783 Cell Signaling Technologies (Danvers, MA) 9101 Polyclonal IgG Anti–β-actin−peroxidase antibody (control for in vitro experiments) 27831566 Sigma (St. Louis, MO) A3854 AC-15 Anti-GAPDH antibody (control for in vivo experiments) 28761447 Cell Signaling Technologies 2118 14C10 Anti-desmin antibody 23308088 Dako (Agilent; Santa Clara, CA) M0760 D33 Open in a separate window AKAP, A-kinase anchoring protein; ERK, extracellular signal–regulated kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ID, identification; MAPK, mitogen-activated protein kinase; PKC, protein kinase C. Antibodies Used in This Study Coimmunoprecipitation Coimmunoprecipitation of proteins from cells or tissue extracts was performed, as published by us previously.

Techniques: Scaffolding, Activity Assay